Description
The human MBL ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 4 hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. After activation, samples and standards are captured by solid bound mannan. Biotinylated tracer antibody will bind to captured MBL. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the MBL standards (log). The MBL concentration of samples, which are run concurrently with the standards, can be determined from the standard curve